Pengaruh Hasil Fraksinasi Ekstrak Etanol Daun Lado-lado (Litsea cubeba,Pers) Terhadap Kadar Asam Urat Serum Darah Mencit Putih Jantan Tinggi Asam Urat

  • Rizky Yulion P
  • Suhatri Suhatri
  • Helmi Arifin

Abstract

Uric acid is the end product of purine metabolism in humans and imbalance of uric acid levels in the blood to triggered hyperuricemia then incidence and prevalence is quite high in Indonesia. This study was conducted to determine whether hyperuricemic affects the decrease of uric acid level in white male mouse hyperurisemia by using lado-lado (Litsea cubeba,Pers) leaf. Sample was extracted with 70% ethanol and subsequently fractionated by using hexane, ethyl acetate and water residual solvents. potassium oxonate doses 250 mg/kgBW and long bean juice (Vigna unguiculata,Walp) doses 0.5 mL/20 gramBB frequency 3 times daily as inducer. It taked for 5 days to hyperuricemia condition. At the time of treatment, only use long peanut juice for inducer. Doses 12,5 mg/kgBW; 25 mg/kgBW and 50 mg/kgBW and variations of 7, 14 and 21 days on hyperuricemic condition. Serum uric acid levels were measured by using a clinical photometer with an enzymatic principle. The resultant decrease in uric acid levels was best obtained at the fraction of ethyl acetate of lado-lado leaves (<0.05) and the doses of 50 mg/kgBW with 7 days of administration was the best variation to decrease uric acid levels in high uric acid serum level condition.

References

A. G. & L. Goodman, Goodman & Gilman’s : Manual of Pharmacology and Therapeutics, 11th ed. San Diego, CA, United States of America: The McGraw - Hill Companies, Inc, 2008.

A. Suhendi, Nurcahyanti, Muhtadi, and E. Sutrisna, “Antihyperurisemia activity of water extract of black seed (Coleus ambonicus Lour) in balb-c mice and its standardization,” Maj. Farm. Indones., vol. 22, no. 2, pp. 77–84, 2011.

A. W. Ningdyah, A. H. Alimuddin, and A. Jayuska, “Uji Toksisitas Dengan Metode BSLT (Brine Shrimp Lethality Test) Terhadap Hasil Fraksinasi Ekstrak Buah Tampoi (Baccaurea macrocarpa),” J. Kim. Khatulistiwa, vol. 4, no. 1, pp. 75–83, 2015.

Badan Pusat Statistik, “Geografis Kabupaten Lima Puluh Kota,” BPS Kabupaten Lima Puluh Kota, 2017. [Online]. Available: https://limapuluhkotakab.bps.go.id/linkTabelStatis/view/id/3. [Accessed: 19-Oct-2017].

B. Lin et al., “Inhibitory effects of the root extract of Litsea cubeba (lour.) pers. on adjuvant arthritis in rats,” J. Ethnopharmacol., vol. 147, no. 2, pp. 327–334, 2013.

B. Tahir, C. Saleh, and S. P. Pasaribu, “Uji Fitokimia, Toksisitas Dan Aktivitas Antioksidan Alami Daun Tumbuhan Kelakai (Stenochlaena palustris) Dengan Metode DPPH,” in Prosiding Seminar Nasional Kimia 2013, 2013, pp. 141–146.

D. A. Juwita, H. Arifin, and Popy Handayani, “Pengaruh Fraksi Air Herba Seledri (Apium graveolens L.) Terhadap Kadar Asam Urat Mencit Putih Jantan Hiperurisemia,” in Seminar Nasional dan Workshop “Perkembangan Terkini Sains Farmasi dan Klinik IV,” 2014, pp. 187–191.

Departemen Kesehatan, Farmakope Herbal Indonesia, Edisi 1. Jakarta: Departemen Kesehatan, 2008.

Departemen Kesehatan, Parameter Standar Umum Ekstrak Tumbuhan Obat. Jakarta: Departemen Kesehatan, 2000.

Dinas Kesehatan RI, Materia Medika Indonesia, vol. 4, no. 1. Jakarta: Direktorat Jendral Pengawasan Obat dan Makanan, 1980.

D. W. Hawking and D. W. Rahn, Pharmacotherapy: A Pathophysiologic Approach, 6th Editio. United States of America: The McGraw-Hill Companies, Inc., 2005.

E. Kumalasari and N. Sulistyani, “Aktivitas Antifungi Ekstrak Etanol Batang Binahong (Anredera Cordifolia (Tenore) Steen.) Terhadap Candida Albicans Serta Skrining Fitokimia,” J. Ilm. Kefarmasian, vol. 1, no. 2, pp. 51–62, 2011.

E. Sutrisna, A. S. Wahyuni, and L. A. Setiani, “Efek Infusa Daging Buah Mahkota Dewa (Phaleria macrocarpa (Sceff.) Boerl.) Terhadap Penurunan Kadar Asam Urat Darah Mencit Putih Jantan Yang Diinduksi Dengan Potassium Oxonate,” J. Chem. Inf. Model., vol. 53, no. 9, pp. 1689–1699, 2013.

F. M. Sholihah, “Diagnosis and Treatment Gout Arthritis,” J Major., vol. 3, no. 7, pp. 39–45, 2014.

H. Arifin, M. Fahrefi, and S. Dharma, “Pengaruh Fraksi Air Herba Seledri (Apium graveolens L.) Terhadap Kadar Kolesterol Total Mencit Putih Jantan Hiperkolesterol,” in Prosiding Seminar Nasional Perkembangan Terkini Sains Farmasi dan Klinik III, 2013, pp. 293–304.

H. Arifin, N. Anggraini, D. Handayani, and R. Rasyid, “Standarisasi Ekstrak Etanol Daun Eugenia Cumini Merr .,” J. Sains Tek. Far., vol. 11, no. 2, pp. 88–93, 2006.

H. G. Vogel, Drug Discovery and Evaluation: Pharmacological Assays, Third., vol. 53. New York: Springer-Verlag Berlin Heidelberg, 2008.

H. L. Putri, R. Retnowati, and Suratmo, “Fraksi n-heksana Dari Ekstrak Metanol Daun Mangga Kasturi (Mangifera casturi Koesterm) Dan Uji Fitokimia,” Kim. Student J., vol. 1, no. 1, pp. 772–777, 2015.

I. Djajanegara and P. Wahyudi, “Pemakaian sel HeLa dalam uji sitotoksisitas fraksi kloroform dan etanol ekstrak daun Annona squamosa,” J. Ilmu Kefarmasian Indones., vol. 7, no. 1, pp. 7–11, 2009.

I. M. O. A. Parwata, W. S. Rita, and R. Yoga, “Isolasi dan uji antiradikal bebas minyak atsiri pada daun sirih (Piper betle Linn) Secara Spektroskopi Ultra Violet-Tampak,” J. Kim., vol. 3, no. 1, pp. 7–13, 2009.

J. Huang, S. Wang, M. Zhu, J. Chen, and X. Zhu, “Effects of Genistein , Apigenin , Quercetin , Rutin and Astilbin on serum uric acid levels and xanthine oxidase activities in normal and hyperuricemic mice,” Food Chem. Toxicol., vol. 49, no. 9, pp. 1943–1947, 2011.

J. . Harborne, Metode Fitokimia, Penentun Cara Modern Menganalisis Tumbuhan, 2nd ed., no. 2. Bandung: ITB, 1987.

J. K. Hwang, E. M. Choi, and J. H. Lee, “Antioxidant activity of Litsea cubeba,” Fitoterapia, vol. 76, no. 7–8, pp. 684–686, 2005.

Kementrian Kehutanan, Kilemo (Litsea cubeba L Persoon), Publikasi., vol. 4. Bogor: Balai Penelitian Teknologi Perbenihan Tanaman Rutan, 2014.

K. R. Sathisha, S. A. Khanum, J. N. N. Sharath, F. Ayisha, and S. Balaji, “Bioorganic & Medicinal Chemistry Synthesis and xanthine oxidase inhibitory activity derivatives,” Bioorg. Med. Chem., vol. 19, no. 1, pp. 211–220, 2011.

L. Hamzah, H. Arifin, and A. Ahmad, “Pengaruh Ekstrak Etanol Rambut Jagung (Zea Mays, L) Terhadap Kadar Asam Uratdarah Mencit Putih Jantan Hiperurisemia,” in Perkembangan Terkini Sains Farmasi dan Klinik IV, 2014, pp. 282–293.

L. Mariana, Y. Andayani, and R. Gunawan, “Analisis Senyawa Flavonoid Hasil Fraksinasi Ekstrak Diklorometana Daun Keluwih (Artocarpus camansi),” vol. 6, no. 2, pp. 50–55, 2013.

L. Scotti et al., “Computer-Aided Drug Design Studies in Food Chemistry,” in Natural And Artificial Flavoring Agents And Food Dyes, vol. 7, Alexandru Mihai Grumezescu and A. M. Holban, Eds. London: Academic Press, 2017, p. 278.

M. F. Daud, E. Sadiyah, and E. Rismawati, “Pengaruh Perbedaan Metode Ekstraksi Terhadap Aktivitas Antioksidan Ekstrak Etanol Daun Jambu Biji (Psidium guajava. L),” Pros. SNaPP2011 Sains, Teknol. dan Kesehat., pp. 55–62, 2011.

Muhtadi, A. Suhendi, N. W., and E. Sutrisna, “Potensi Daun Salam (Syzigium Polyanthum Walp.) Dan Biji Jinten Hitam (Nigella Sativa Linn) Sebagai Kandidat Obat Herbal Terstandar Asam Urat,” J. Chem. Inf. Model., vol. 53, no. 9, pp. 1689–1699, 2013.

M. Sangi, L. Momuat, and M. Kumaunang, “Uji toksisitas dan skrining fitokimia tepung gabah pelepah aren (Arenga pinnata),” J. Ilm. Sains, vol. 12, no. 2, pp. 127–134, 2012.

N. P. R. Artini, S. Wahjuni, and W. D. Sulihingtyas, “Ekstrak Daun Sirsak (Annona muricata L.) Sebagai Antioksidan Pada Penurunan Kadar Asam Urat Tikus Wistar,” J. Kim., vol. 6, no. 2, pp. 127–137, 2012.

P. Bentley, Memorizing Medicine A Revision Guide. London, UK: Royal Society of Medicine Press Ltd Published, 2007.

Q.-H. Hu, C. Wang, J.-M. Li, D.-M. Zhang, and L.-D. Kong, “Allopurinol, rutin, and quercetin attenuate hyperuricemia and renal dysfunction in rats induced by fructose intake: renal organic ion transporter involvement.,” Am. J. Physiol. Renal Physiol., vol. 297, no. 17, pp. F1080-91, 2009.

S. H. Nile and S. won Park, “Activity Directed Screening, Antioxidant Activity And Xanthine Oxidase Inhibition By Bioactive Compounds From Cudrania Tricuspidata,” in International Conference on Medicinal and Herbal Products, 2017, no. January 2013, p. 8.

S. Lin, G. Zhang, Y. Liao, J. Pan, and D. Gong, “Dietary Flavonoids as Xanthine Oxidase Inhibitors: Structure-Affinity and Structure-Activity Relationships,” J. Agric. Food Chem., vol. 63, no. 35, pp. 7784–7794, 2015.

S. Y. Wang, C. W. Yang, J. W. Liao, W. W. Zhen, F. H. Chu, and S. T. Chang, “Essential oil from leaves of Cinnamomum osmophloeum acts as a xanthine oxidase inhibitor and reduces the serum uric acid levels in oxonate-induced mice,” vol. 15, pp. 940–945, 2008.

U. Takahama, Y. Koga, S. Hirota, and R. Yamauchi, “Inhibition of xanthine oxidase activity by an oxathiolanone derivative of quercetin,” Food Chem., vol. 126, no. 4, pp. 1808–1811, 2011.

Y. S. Song, S. H. Kim, J. H. Sa, C. Jin, C. J. Lim, and E. H. Park, “Anti-angiogenic, antioxidant and xanthine oxidase inhibition activities of the mushroom Phellinus linteus,” J. Ethnopharmacol., vol. 88, no. 1, pp. 113–116, 2003.
Published
2017-12-28
How to Cite
P, Rizky Yulion; SUHATRI, Suhatri; ARIFIN, Helmi. Pengaruh Hasil Fraksinasi Ekstrak Etanol Daun Lado-lado (Litsea cubeba,Pers) Terhadap Kadar Asam Urat Serum Darah Mencit Putih Jantan Tinggi Asam Urat. Jurnal Sains dan Teknologi Farmasi, [S.l.], v. 19, n. Supl1, p. s96-s103, dec. 2017. ISSN 2580-5959. Available at: <http://jstf.ffarmasi.unand.ac.id/index.php/jstf/article/view/107>. Date accessed: 18 nov. 2018.

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