Analisis α-Mangostin Dalam Minuman Herbal Kulit Buah Manggis (Garcinia mangostana L.) dengan Metode Kromatografi Lapis Tipis-Densitometri

  • Regina Andayani
  • Friardi Friardi


These days, mangosteen juice is becoming a popular “herbal drink.” Some marketers claim that mangosteen juice can treat diarrhea, menstrual problems, urinary tract infections, tuberculosis, and a variety of other conditions. However, there is no reliable scientific evidence to support these claims.  This study aims to determination of α-mangostin in the herbal drinks of mangosteen rind on the market by thin layer chromatography-densitometric method. The analysis was performed with TLC Scanner using Camag TLC Scanner 4 with Wincats software, the maximum wavelength of α-mangostin was 316 nm, viewed using 254 nm and 366 nm UV Camag lamps, Camag Nanomat 4, Camag capillary pipe size 2 μL, Camag Twin Chamber, silica gel plate 60 F254 (Merck) size 20 x 20 cm. The mobile phase used was chloroform and ethyl acetate (9: 1). After being seen under a 254 nm UV lamp, the α-mangostin standard, samples B and C have the same Rf value of 0.48. The concentration series used 50, 100, 150, 300 and 400 ppm yield regression equation y = 1326,1658 + 19,7647x with correlation coefficient (r) = 0,99415. This correlation coefficient shows a linear result. Limit detection (LOD) and quantification limit (LOQ) obtained were 54.38 μg/mL and 181.28 μg/mL. The percentage of recovery obtained from the analysis of 90.04%, this validation method gives accurate and precise results (intraday precision value RSD / Relative Standard Deviation was ≤ 1.28%, while the interday precision test was ≤ 2.39%). The average result obtained for samples B and C were 0.040% w/v and 0.118% w/v, respectively. This research is cheap, accurate, precise, linear and fast. The method of TLC-densitometry meets the validation criteria for the quantitative determination of α-mangostin in the herbal drinks of mangosteen rind.


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How to Cite
ANDAYANI, Regina; FRIARDI, Friardi. Analisis α-Mangostin Dalam Minuman Herbal Kulit Buah Manggis (Garcinia mangostana L.) dengan Metode Kromatografi Lapis Tipis-Densitometri. Jurnal Sains dan Teknologi Farmasi, [S.l.], v. 19, n. Supl1, p. s23-s28, dec. 2017. ISSN 2580-5959. Available at: <>. Date accessed: 23 mar. 2018.